Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Nov 10;66:83–93. doi: 10.1016/j.yjmcc.2013.11.001

Fig 5.

Fig 5

Biased PKIα expression may reduce nuclear PKA sensitivity to ISO stimulation. A, Simulated AKAR-NLS responses to 1 μmol/L ISO with increasing nuclear phosphatase expression. B, Model predicts nuclear phosphatase inhibition does not recover AKAR-NLS’s ISO sensitivity. C, Experimental validation for model simulations. Pre-treatment with 10 nmol/L Calyculin A does not alter nuclear AKAR-NLS sensitivity to ISO (n ≥ 9 cells each). D, Model predicts nuclear PKA ISO sensitivity requires biased PKI expression. E, Immunofluorescence labeling with anti-PKIα antibodies identify nearly exclusive nuclear PKIα expression.