. Author manuscript; available in PMC: 2014 Feb 18.

Published in final edited form as: Anal Chim Acta. 2011 Mar 11;693(0):106–113. doi: 10.1016/j.aca.2011.03.011

Table 3.

The mobility of IPA and CG-Ab on seven types of membranes^a

							1% BSA Blocking^b
	No blocking
				5min			15min			1hr
	Rf		Flow time (s) of solvent	Rf		Flow time (s) of solvent	Rf		Flow time (s) of solvent	Rf		Flow time (s) of solvent
	IPA	CG-Ab		IPA	CG-Ab		IPA	CG-Ab		IPA	CG-Ab
1 Whatman NC (RP)	0~0.65	0.15	25	0~0.60	0~0.30	110	0~0.50	0~0.30	120	0~0.40	0.5~0.70	116
2 UltraBind	0~0.75	0~0.20	45	0~0.80	0~1.0	90	0~0.90	0~1.0	131	0~0.90	0.5~1.0	98
3 Immunodyne ABC	0~0.25	nm	253	0~0.30	0~0.40	229	0~0.30	0~0.40	233	0~0.30	0~0.45	239
4 Biodyne A	0~0.28	nm	110	0~0.27	0.02	92	0~0.40	0~0.20	120	0.20~0.30	0~0.70	117
5 Biodyne B	0~0.25	nm	122	0~0.25	nm	238	0.25~0.35	nm	256	0.25~0.35	nm	229
6 Hybond N⁺	0~0.10	nm	67	0~0.25	nm	347	0~0.10	nm	321	0~0.20	nm	319
7 Immobilon	Nm	nm	nm	nm	nm	nm	nm	nm	nm	nm	nm	nm

Each membrane strip was a regular square of 3.0 × 2.0 cm. One microliter of IPA and CG-Ab was pipeted at 0.2 cm from the starting point. The reagents were developed by soaking the membranes with 10% methanol-PBST The R_f and flow time were measured when the solvent reached 2.2 cm from the starting point. “nm” means no migration.

Membranes were blocked by incubation with 1% BSA for indicated time.