Figure 4.

Mitochondrial DNA biogenesis is increased in sciatic nerves of Gdpa1^−/− mice. (A) DNA of the indicated tissues was isolated from 19-month-old Gdap1^−/− and wild-type mice. The relative amount of mitochondrial DNA (mtDNA) in relation to genomic DNA (gDNA) was determined by quantitative PCR. (B) Total RNA was isolated from sciatic nerve (SCN) and spinal cord (SPC) lysates from 19-month-old Gdap1^−/− and wild-type mice. The relative expression levels of nuclear factors involved in mitochondrial biogenesis and redox protection were determined by quantitative PCR in relation to β-actin levels. (A and B) Graphs represent means and standard error of tissues of n = 3 animals per genotype, n.s. = not significant, *P < 0.05.