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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Cardiovasc Toxicol. 2013 Mar;13(1):77–84. doi: 10.1007/s12012-012-9180-0

Figure 1.

Figure 1

Real-time PCR measurement of CYP expression. Data are normalized to GAPDH and PBS injection into the yolk. A. CYP1A4 expression in HH17 hearts. B. CYP1A4 expression in HH17 extra-cardiac tissue. CYP1A4 expression was not significantly altered relative to total RNA (GAPDH) in either HH17 hearts or extra-cardiac tissue at PBS, 8 or 800 ppb TCE exposure. C. CYP2C45 expression in HH17 hearts. D. CYP2C45 expression in HH17 extra-cardiac tissue. CYP2C45 expression was not significantly altered relative to total RNA (GAPDH) in either HH17 hearts or extra-cardiac tissue after PBS, 8 or 800 ppb TCE exposure. E. CYP2H1 expression in HH17 hearts. F. CYP2H1 expression in HH17 extra-cardiac tissue. CYP2H1 expression was significantly increased relative to total RNA (GAPDH) in HH17 hearts after 8 ppb TCE exposure (* = p-value < 0.0001, One-way ANOVA), while PBS and 800 ppb TCE exposure showed no significant change in expression. Real-time PCR results for CYP2H1 showed no significant change in expression at PBS, 8 or 800 ppb TCE exposure.