Table 2. Multivariate generalized linear model* analysis.

Genes (% methylation)	EN1 (n = 87)		PCDH10 (n = 86)		RSPO2 (n = 86)		ZIC1 (n = 87)		ZNF610 (n = 86)
	Mean + SE	P	Mean + SE	P	Mean + SE	P	Mean + SE	P	Mean + SE	P
Area
Low risk	20.5 ± 0.7	Ref	25.2 ± 0.9	Ref	15.4 ± 0.7	Ref	11.8 ± 0.7	Ref	14.4 ± 1.0	Ref
High risk	24.1 ± 0.8	0.003	29.1 ± 0.9	0.003	18.9 ± 0.8	0.002	15.4 + 0.8	0.002	18.8 ± 1.0	0.005

Diagnosis
Normal/NAG	21.7 ± 0.8	Ref	26.5 ± 0.9	Ref	16.6 ± 0.8	Ref	13.3 ± 0.8	Ref	17.2 ± 1.1	Ref
MAG	22.8 ± 1.4	0.51	27.8 ± 1.6	0.51	17.8 ± 1.4	0.44	13.8 ± 1.3	0.79	16.3 ± 1.8	0.69
IM/dysplasia	22.7 ± 0.9	0.47	27.6 ± 1.1	0.47	17.4 ± 0.9	0.53	13.8 ± 0.9	0.71	15.6 ± 1.2	0.37

H. pylori genotypes
Uninfected	16.5 ± 1.8	Ref	15.2 ± 2.1	Ref	9.4 ± 1.8	Ref	6.8 ± 1.8	Ref	9.9 ± 2.5	Ref
cagA negative	19.4 ± 1.5	0.214	23.7 ± 1.7	0.002	14.5 ± 1.5	0.024	11.2 ± 1.4	0.05	11.8 ± 2.0	0.53
cagA positive	23.5 ± 0.6	0.001	29.3 ± 0.7	0.000	18.5 ± 0.6	0.000	14.9 ± 0.6	0.000	18.1 ± 0.8	0.003

Chronic Inflammation†
1st Quartile (MN)	21.9 ± 0.69	Ref	25.9 ± 0.78	Ref	16.1 ± 0.66	Ref	12.5 ± 0.64	Ref	15.8 ± 0.92	Ref
3rd Quartile (MN)	22.7 ± 0.78	0.470	28.6 ± 0.88	0.020	18.2 ± 0.75	0.036	15.0 ± 0.75	0.003	17.3 + 1.04	0.288

^*Multivariate model adjusted for age and including risk area, diagnosis and H. pylori genotypes; ^†Multivariate model adjusted for risk area and H. pylori genotypes; interquartile range comparison; Ref, Referent category; SE, standard error; MN, mononuclear cells