Table 1.

Primer gene sequence for the assessed genes, their size and annealing temperatures.

Genes	Primer sequence (5′–3′) (forward/reverse)	Product size (bp)	Annealing temperature (°C)
OPN	Forward: G A C A G C C A G G A G A A G G A C A G	169	50
	Reverse: T C T T C A C T C T T C G G C T C G A T
OCN	Forward: G T G C A G A G T C T G G C A G A G G	153	50
	Reverse: G G T T G A G C T C G C A C A C C T
GAPDH	Forward: T G C C C T G G G G T G G G A A T G G A	200	45
	Reverse: A G G G G T G A G G G A C A C G A G G C

OPN: osteopontin; OCN: osteocalcin; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.