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. 2013 Oct 10;4:2041731413508668. doi: 10.1177/2041731413508668

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© The Author(s) 2013

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 2. — Decellularization of osteogenic differentiated MSC–collagen constructs with detergent at different dosages. (a–d) Routine H&E staining (scale bar: 100 µm); (e–h) SEM images; (a and e) without decellularization; (b and f) decellularized with 4% sodium deoxycholate; (c and g) decellularized with 5% sodium deoxycholate; (d and h) decellularized with 6% deoxycholate; (i) DNA content after decellularization (*statistical significant difference: p = 0.05); (j) calcium content per dry weight after decellularization (n = 3, each with duplicates).

MSC: mesenchymal stem/stromal cell; H&E: hematoxylin and eosin; SEM: scanning electron microscope.