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. 2014 Jan 28;6(1):35–47. doi: 10.18632/aging.100632

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Copyright: © 2014 Pazienza et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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Overexpression of macroH2A1 isoforms (macroH2A1.1 or macroH2A1.2) and lipid accumulation in Hepa1-6 cells and HepG2 cells. Upper panels: cells were transiently transfected with Lipofectamine with either an empty vector (control, CTL) or with Cherry-tagged macroH2A1.1 and macroH2A1.1 constructs. 24 hours post transfection cells were exposed to a 100 mM 1:1 mixture of oleic acid/linoleic acid (FFA) conjugated with albumin, for an additional 24 hours. Cells were then fixed, nuclei stained with DAPI (blue) and lipids with ORO. Overlay of Cherry tagged macroH2A1-transfected nuclei and DAPI staining is observed in pink. Lower panel: quantifications of ORO stained areas are means ± SEM of 1000 cells per condition. *p<0.05.