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. Author manuscript; available in PMC: 2014 Feb 18.
Published in final edited form as: Nat Protoc. 2013 May 2;8(6):1028–1041. doi: 10.1038/nprot.2013.049

Figure 2.

Figure 2

Stem cell types that can be derived and propagated in culture representing the three blastocyst lineages. Embryonic stem (ES) cells represent the epiblast, trophoblast stem (TS) cells represent the trophectoderm and extraembryonic endoderm (XEN) cells represent the primitive endoderm cell lineage. Heterogeneities in XEN cell morphology are indicated: highly refractile phase-bright and epithelial-like. Cognate embryo– derived stem cells retain the expression of key lineage-associated genes. GF, growth factor; iPS, induced pluripotent stem; OKSM, Oct4, Sox2, Klf4 and c-Myc. Scale bars, 100 µm.