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. Author manuscript; available in PMC: 2014 Feb 18.
Published in final edited form as: J Am Chem Soc. 2013 Oct 25;135(44):16397–16409. doi: 10.1021/ja405239v

Figure 8.

Figure 8

SDS-PAGE gel electrophoresis and Western blotting of Aβ42 species. (a) SDS-PAGE gel of FAM- Aβ42 alone (lane 1), CuSO4 (lane 2), CuSO4 + curcumin (lane 3), CuSO4 + CRANAD-17 (lane 4), and CuSO4 + CRANAD-58 (lane 5). (b) Quantitative analysis of the intensities of the monomeric bands in (a). (c) SDS-PAGE gel of FAM- Aβ42 treated with CuSO4 + imidazole control (lane 1–3, ratio FAM- Aβ42/ imidazole control = 1:10, 1:5, 1:1), CuSO4 + CRANAD-17 (lane 4–6, ratio FAM- Aβ42/CRANAD-17 = 1:10, 1:5, 1:1), and CuSO4 only (lane 7); (d) Western blotting of the native Aβ42 (lane 1), with CuSO4 (lane 2), CuSO4 + curcumin (lane 3), CuSO4 + CRANAD-17 (lane 4), and CuSO4 + CRANAD-58 (lane 5); (e) Quantitative analysis of the monomeric bands in (d); (f) Dose dependent study for CRANAD-17. Quantitative analysis of the native Aβ42 monomeric bands (without copper), with CuSO4, and with CuSO4 + CRANAD-17 (Aβ42/CRANAD-17 = 1:1, 1:5, and 1:10). The data indicated that the attenuation of copper-induced crosslinking by CRAND-17 was concentration dependent.