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Immunoassay: Quantification of secreted proteins. Secretion of (a) OPG, (b) VEGFA and (c) OC to cell culture medium from scaffolds with 10 nM and 10 µM SIM is shown in percentage of control, scaffolds without SIM, at 2, 8, 14 and 21 days. Values represent the mean ± SD. Statistical analysis: (a) p ≤ 0.05 versus alginate-coated scaffold without SIM.
SIM: simvastatin; OPG: osteoprotegerin; OC: osteocalcin; VEGFA: vascular endothelial growth factor A; SD: standard deviation.
