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. 2013 Nov 26;4:2041731413515670. doi: 10.1177/2041731413515670

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© The Author(s) 2013

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 9. — Confocal laser scanning microscopy visualization of type I collagen deposition in alginate-coated TiO₂ scaffolds with or without simvastatin (SIM). Fluorescence immunocytochemical analysis of type I collagen in primary human osteoblasts cultured on alginate-coated TiO₂ scaffolds. Type I collagen is detected in the majority of the cells cultured on scaffolds with (a) 10 nM SIM, (b) 10 µM SIM and (c) without SIM. Extracellular collagen fibrils are only seen in scaffold (c) without SIM. Type I collagen (red), DNA (blue), TiO₂ scaffold surface (white).