Table 1.

Primer sequences used for real-time RT-PCR assays.

Gene	Sequence of left primer	Sequence of right primer
GAPDH	CTCTGCTCCTCCTGTTCGAC	ACGACCAAATCCGTTGACTC
COL1A1	CATCTCCCCTTCGTTTTTGA	CCAAATCCGATGTTTCTGCT
ALPL	GACAAGAAGCCCTTCACTGC	AGACTGCGCCTGGTAGTTGT
BMP2	TCAAGCCAAACACAAACAGC	AGCCACAATCCAGTCATTCC
TNFRSF11B	TGGGAGCAGAAGACATTGAA	GTGTCTTGGTCGCCATTTTT
VEGFA	TCTTCAAGCCATCCTGTGTG	ATCTGCATGGTGATGTTGGA
BGLAP	GCAAGTAGCGCCAATCTAGG	GCTTCACCCTCGAAATGGTA

RT-PCR: reverse transcriptase–polymerase chain reaction; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; COL1A1: collagen type I alpha 1; ALPL: alkaline phosphatase; BMP2: bone morphogenetic protein 2; TNFRSF11B: osteoprotegerin; VEGFA: vascular endothelial growth factor A; BGLAP: osteocalcin.