Figure 3.

Effects of farnesylthiosalicylic acid (FTS) on T lymphocyte subsets in adjuvant-induced arthritis (AIA). Spleens, inguinal lymph nodes (ILNs) and sera of FTS-treated and vehicle-treated rats with AIA were excised on day 16 after disease induction. a. Spleens (upper panel) and ILNs (middle panel) were assayed for CD4⁺ and CD8⁺ T cells by flow cytometry. Statistical analysis of the flow cytometry results (n = 10) is presented (lower panel). (b,c) ILNs were tested for forkhead box protein 3 (FoxP3) expression by Western blotting (b) and flow cytometry (c), as described in the Methods section. Representative blots and flow cytometry are shown in the left panels and the statistical analysis in the right panels. *P < 0·05; **P < 0·01; ***P < 0·001 compared to vehicle-treated controls, Student's t-test.