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. 2014 Feb 4;175(3):476–484. doi: 10.1111/cei.12229

Figure 1.

Figure 1

Kissper controls calcium release and reactive oxygen species (ROS) production in intestinal epithelial cells. (a) Immunofluorescence of intracellular ROS [chloromethyl-2′7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA, top panel] or a Wallac 1420 multi-label counter (a, bottom line) DCF, 5-(and-6)CM-H2DCFDA). Values are means ± standard error (SE), n = 6. Asterisks indicate that means differ from samples cultured with medium. *P < 0·05. (b) Caco-2 cells were loaded with Fura-2AM. [Ca2+] imaging pseudocolour ratiometric images. Colours correspond to the scale of [Ca2+]i increase. Red, high [Ca2+]i contents. (b, bottom line) Quantification of results from three independent experiments is shown in the histogram. Values are means ± standard error (s.e.). Asterisks indicate that means differ from samples cultured with medium alone. *P < 0·05.