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. 2014 Feb 14;5:2041731414524758. doi: 10.1177/2041731414524758

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© The Author(s) 2014

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 1. — Evidence for myogenic induction. MSCs were treated with 5-aza-CdR for 24 h and then cultured for 2 weeks to induce myogenic differentiation. (a–d) Immunostaining and flow cytometry on day 14. Compared with untreated MSCs (0 µM), 5-aza-CdR-treated MSCs exhibited upregulated expression of the myogenic-specific proteins desmin (a, b) and MyHC (c, d), suggesting that the 5-aza-CdR-treated MSCs acquired characteristics of myogenic cells. Myotube formation (arrow) was observed in 5-aza-CdR-treated MSCs, but not undifferentiated MSCs. DAPI is the nuclear staining. Flow cytometry: filled blue shows isotype control and empty green, corresponding antibody. Numbers represent percentages of cells staining positive for the given antibody. Data are representative of two independent experiments.

MSC: mesenchymal stem cell; 5-aza-CdR: 5-aza-2′-deoxycytidine; DAPI: 4,6-diamidino-2-phenylindole; MyHC: myosin heavy chain.