Figure 4.

Induction of regulatory T cells in response to undifferentiated and 5-aza-CdR-treated MSCs. (a–c) After 5 days of coculture, splenocytes cocultured with MSCs were stained with antibodies against CD4, CD25, and Foxp3 and assessed by flow cytometry. Addition of undifferentiated MSCs and 5-aza-CdR-treated myogenic-induced MSCs dose dependently increased the proportion of (a) CD4+Foxp3+, (b) CD4+CD25+, and (c) CD25+Foxp3+ expression in splenocyte culture by flow cytometry. Numbers represent percentages of cells staining positive for the given antibody. Data are representative of two independent experiments with the same pattern of results.

5-Aza-CdR: 5-aza-2′-deoxycytidine; MSC: mesenchymal stem cell; PE: phycoerythrin; FITC: fluorescence isothiocyanate; APC: allophycocyanin.