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. 2014 Jan 2;101(3):364–372. doi: 10.1093/cvr/cvt342

Figure 5.

Figure 5

Hyperglycaemia impairs VEGFR2 expression in simulated ischaemia (hypoxia and nutrient deprivation) without altering its mRNA expression. (A) In simulated ischaemia, VEGFR2 protein expression is impaired in HUVECS pre-cultured in high glucose (25 mM or H Gluc) compared with HUVECS cultured in normal glucose (5 mM or N Gluc). (B) Quantization of the bands in A, n = 5/grp, **P < 0.01. (E) In simulated ischaemia, VEGFR2 mRNA by quantitative RT-PCR showed normal expression in HUVECS cultured in high glucose prior to simulated ischaemia exposure (n = 6/grp, NS = not significant, P = 0.33). (D) Insulin treatment did not increase VEGFR2 expression in HUVECs. Cells were cultured in the presence or absence of insulin, and then exposed to simulated ischaemia for 12, 24, 48, and 72 h following an initial overnight starvation in insulin and growth factor-free medium. A representative western blot of VEGFR2 expression following treatment with 10 nM of insulin at 72 h is shown. (E) Quantization of the blot in D (n = 3/grp, NS = not significant, P > 0.05).