Figure 2. Reduction of Btk/Slp65-mediated pre-BCR signaling is associated with progressive loss of Igκ GLT.

Quantitative RT-PCR analysis for κ⁰ (A), λ⁰ (B), and V_κ GLT (C) of FACS-sorted B220⁺CD19⁺ pre-B/pro-B cell fractions from the indicated mice on a VH81x transgenic Rag1 ^−/− background. Expression levels were normalized to those of Gapdh, whereby the values in WT pre-B cells were set to one. Bars represent mean values and error bars denote standard deviations for four independent mice per group.