Figure 6. Long-range chromatin interactions of κ regulatory elements correlate with TF binding and histone modifications.

(A-D) For fragments within the V_κ region, average 3C-seq interaction frequencies were calculated for fragments that did (+) or did not (−) contain binding sites for TFs or H3K4 histone modifications (as determined by previous ChIP-Seq studies; see Materials and Methods for references). Data for the three viewpoint and the five B-cell precursor fractions representing a pre-BCR signaling gradient are shown for Ctcf (A), Ikaros (B), E2a (C), and H3K4 di- and tri-methylation (Me2/3). Statistical significance was determined using a Mann–Whitney U test (*p<0.05; **p<0.01; ***p<0.001; n.s., not significant, p≥0.05).