Figure 6. LPS or CpG treatment reduces the number of ciprofloxacin-tolerant bacteria in the cLN.

C57BL/6 mice were infected for 1 d or 3 d with wt S. Tm (5×10⁷ cfu by gavage). Ciprofloxacin treatment (2×62 mg/kg/d) was started at day 1 p.i. and continued until the end of the experiment, as indicated. The indicated groups were treated with a single dose of LPS (5 µg intraperitoneally) or CpG (100 µg intraperitoneally) at day 2 p.i. Cfus in the cLN were analyzed by plating. For gut content and spleen loads, see Figure S14. Each symbol represents one mouse. Dashed line, detection limit. p, nonparametric statistical analysis (Mann–Whitney U test). n.s., not significant.