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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Gastroenterology. 2013 Jan 24;144(5):1076–1085.e2. doi: 10.1053/j.gastro.2013.01.041

Figure 3. (A) Histologic features characteristic of chronic pancreatitis were similar in all experimental CP groups.

Figure 3

Representative hematoxylin and eosin-stained sections (100×) showing moderate acinar loss, fibrosis, microscopic duct dilatations, tubular complexes and inflammatory infiltrate WT, T−/− and CB−/− CP groups (N=16-20/group). (B) Fibrosis: Sirius red-stained sections (100×) under fluorescence showing marked fibrosis in CP groups. Quantification (C) confirmed comparable fibrosis in WT, T−/− and CB−/− CP groups (P<.0001 pair wise for each CP group vs control, not significant pair wise among CP groups, N=16-20/group). (D,E) Comparable overexpression of collagen-1, desmin and αSMA in CP groups. Representative western blots are shown in (D) and their quantification is shown as box-whisker plots with outliers in (E). N=5-8/group for each protein. Control vs WT, T−/−, CB−/− CP groups respectively: P= .0008, .001, .04 for collagen-1; .001, .001, .009 for desmin and .001, .001, .009 αSMA. P-values not significant for all pair wise comparisons among CP groups. Vimentin staining confirmed marked increase in stellate cell population in the CP groups (F). Representative sections (200×) stained with vimentin are shown.