Table 1.
Primer and adaptor sequences
| Primer/adaptor | Sequence (5′-3′) | Application |
|---|---|---|
| Adaptor EcoRI-f |
CTCGTAGACTGCGTACC |
Ligation |
| Adaptor EcoRI-r |
AATTGGTACGCAGTCTAC |
Ligation |
| Adaptor MseI-f |
GACGATGAGTCCTGAG |
Ligation |
| Adaptor MseI-r |
TACTCAGGACTCAT |
Ligation |
| EcoRI-0 |
GACTGCGTACCAATTC |
Non-selective PCR |
| MseI-0 |
GATGAGTCCTGAGTAA |
Non-selective PCR |
| 5′DY-EcoRI-AT |
GACTGCGTACCAATTCAT |
Selective PCR |
| 5′DY-EcoRI-AC |
GACTGCGTACCAATTCAC |
Selective PCR |
| MseI-AT |
GATGAGTCCTGAGTAAAT |
Selective PCR |
| MseI-AC |
GATGAGTCCTGAGTAAAC |
Selective PCR |
| EcoRI-AC |
GACTGCGTACCAATTCAC |
Re-amplification PCR |
| EcoRI-AT | GACTGCGTACCAATTCAT | Re-amplification PCR |
Primer sets were designed as reported by Bove et al. [18].