Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Leukemia. 2013 Jun 28;27(12):2366–2375. doi: 10.1038/leu.2013.194

Figure 6. RGB-286638 Effects on miRNA Expression.

Figure 6

(A) RGB-286638 modulated miRNA expression in MM.1S cells alone and in culture with BMSCs. TaqMan® miR-21-3p and miR-19a-5p miRNA Specific Assays using RT-PCR were performed on aliquots of total RNA extracts from MM.1S cells cultured for 8h in control media or 50nM RGB-286638, with or without BMSCs. Data are presented as relative quantity (RQ) values in RGB-286638 treated versus untreated cells. The experiments were carried out in triplicates, (*) if p<0.05 and two (**) if p<0.01.

(B) RGB-286638 suppressed the expression of the oncogenic miR-19a-5p, miR-21-3p, and miR-92a-1-5p in mutant-p53 MM cell lines. U266, OPM1, and RPMI cells were incubated in media alone or in 50nM RGB-286638 for 8h. Total RNA extracts were subjected to TaqMan Specific miRNA Assays for miR-21-3p, -19a-5p, and -92a-1-5p detection. Data is presented as RQ values of RGB-286638-treated versus -untreated cells. Experiments were carried out in triplicates, (*) if p<0.05 and two (**) if p<0.01.