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. 2014 Feb 18;9(2):e87644. doi: 10.1371/journal.pone.0087644

Figure 3. Cell viability of undifferentiated and differentiated ES cells after ultra-quick freezing/thawing.

Figure 3

(A) Morphological assessment of the undifferentiated or the differentiated Ascl1 inducible ES cells (mouse ES cells under Tet-off system). (B) AQP4 mRNA expression in undifferentiated (Undiff.) or differentiated (Diff.) ES cells, assessed by RT-PCR. (C) Cell viability of the undifferentiated or the differentiated ES cells at different concentration of DMSO (3, 5 and 10%) after ultra-quick freezing. Data are shown as the mean ± standard deviation (n = 2–3, *P<0.05).