Table 1. Cells expressing AQP either exogenously or endogenously are resistant to ultra-quick freezing/thawing.
Cell lines | Expression of AQP4 | Cell viability ± S.D. (%) |
CHO | (-) | 2.4±1.4 |
AQP4 | 60.5±16.7 | |
MDCK | (-) | 0.7±0.8 |
AQP4 | 37.2±8.7 | |
Astrocytes | AQP4 KO | 2.5±2.3 |
WT | 54.1±6.4 |
Cell viability after ultra-quick freezing/thawing: comparison between CHO, MDCK cells and stably expressing AQP4 cells (AQP4-CHO, AQP4-MDCK), and also primary culture astrocytes from WT-mice (AQP4+) or AQP4-null mice (n ≧ 3, ***P<0.001).