Figure 4. Effect of E499V mutation on HA synthesis. 293T cells were transfected with empty vector pcDNA3.1 (+), pcDNA3.1-HAS2 wild-type and the E499V mutant separately.

HA content in media was determined as described in the methods section. Results are representative of three separate experiments. The significance of differences was calculated using the independent-samples t test. *p<0.05, **p<0.01 versus empty vector pcDNA3.1 (+); #p<0.05, ##p<0.01 versus wild-type.