A. Transcriptional profile of wt grown in the presence of cantharidin. GO Biological Term Enrichment was applied to significantly (P-value<0.05) downregulated genes (log2 (drug/DMSO) >|2|).
B. qRT-PCR analysis demonstrates that CaCRG1 is a cantharidin-responsive gene. Data are means of at least three independent experimental replicates, and error bars are SD.
C. cacrg1Δ/Δ has reduced adherence to plastic surface in the presence and absence of cantharidin. *P-value <0.05, ** <0.01.
D. cacrg1Δ/Δ fails to form hyphae in the presence of cantharidin.