A. Fitness of double deletion mutants in liquid SC media at 39°C.
B. CaCRG1 interacts with GlcCer genes in a condition-dependent manner. The unexpected phenotypes for double mutants (relative to wt and crg1Δ/Δ mutant) are highlighted: “+” denotes positive genetic interactions, “-“ denotes negative genetic interaction.
C. Adherence of cacrg1Δ/Δsld1Δ/Δ to abiotic surface.
D. Abundance of ceramide-related species in cacrg1Δ/Δ and wt. Statistically significant difference in the abundance (Student’s t-test) is shown in red. Error bars are standard deviation. *P-value<0.05, **P-value<0.1, Student’s t-test.