Table 3. Gastric microbiota studies in mice.
| Study | Method | # of Samples | Age | Sex | Species | HP status | Phyla (%) | Genus information | HP effect |
|---|---|---|---|---|---|---|---|---|---|
| Kabir et al. 1997 | Culture | 3 | 5 wk-old + up to 9 wk infection | Male | BALB/c mice (Japan) | HP infected/no colonization | Firmicutes (99.9%), Proteobacteria (< 0.01%) | Firmicutes (Lactobacillus) = 99.9% and Firmicutes (Enterococcus and Staphylococcus) and Proteobacteria (Enterobacter) = < 0.01% | Blocked by high number of Lactobacillus spp |
| Aebischer et al. 2006 | Dot-blot hybridization, then Sanger-sequenced 16S rRNA library | 1 | 6–8 wk old + 2 mo | Female | BALB/c (Germany) | Uninfected | 60–90% Lactobacillus. 10–40% - other. | ||
| 1 | 6–8 wk old + 2 mo infection | Female | BALB/c (Germany) | HP P76 infected | Firmicutes, Proteobacteria, Bacteroidetes | Levels shift from uninfected | HP increases Firmicutes (Clostridium, Eubacterium, Ruminococcus and Streptococcus), Bacteroidetes (Bacteroides/Prevotella), Proteobacteria (Escherichia). Decreases Lactobaciillus. Effect independent of pH/pathology | ||
| Tan et al. 2007 | Culture and T-RFLP (two sets) | 9–10 mice/group | 6–8 wk old + 0.5, 3 or 6 mo | Female | C57BL/6 (Australia) | Uninfected | Firmicutes (Lactobacillus) = 99.9% | ||
| 9–10 mice/group | 6–8 wk old + 0.5, 3 or 6 mo infection | Female | C57BL/6 (Australia) | HP SS1 infected | No change from uninfected | Cultured Proteobacteria (E. coli, Moraxella osloensis, Actinobacillus muris, Pasteurella spp.) and Firmicutes(Enterococcus faecalis, Lactobacillus spp) | |||
| Lofgren et al. 2011 | 454 pyrosequencing of 16S rDNA amplicons | 2 | 6 wk old + 15 wk | Male | INS-GAS FVB/N (USA) | Uninfected | Firmicutes (55%), Bacteroidetes (25%), Cyanobacteria (15%), Proteobacteria and other (5%) | No H. pylori sequences detected. Observed 175 OTUs | |
| 3 | 6 wk old + 15 wk infection | Male | INS-GAS FVB/N (USA) | HP SS1 infected | Firmicutes (90%), Proteobacteria, Cyanobacteria, Bacteroidetes, and Tenericutes (10%) | Firmicutes (Lactobacillus) dominant | Low H. pylori reads. Increases OTUs in stomach, cecum and colon. Higher pathology. Observed 235 OTUs | ||
| Rolig et al. 2013 | PhyloChip | 5 | 7 wk old + 4 wk | Female | C57BL/6 (Taconic Farms/USA) | Uninfected | Firmicutes (73.4%), Bacteroidetes (11.8%) Verrucomicrobia (8.5%), Proteobacteria (3.2%), Actinobacteria (1.9%) (% of species) | Firmicutes (class Clostridia) 44% | |
| 4 | 7 wk old + 4 wk infection | Female | C57BL/6 (Taconic Farms/USA) | HP SS1 infected | Increases Firmicutes (class Clostridia), Proteobacteria (Helicobacter), Verrumicrobia. Decreased Firmicutes (class Bacilli), Bacteroidetes, Proteobacteria | ||||
| Lertpiriyapong et al. 2013 | qPCR for 3 ASF species | 27 | 7–11 wk old + 7 mo | 13 males, 9 females | INS-GAS FVB/N (USA) | Uninfected | Firmicutes, Bacteroidetes | 3 restricted ASF species | |
| 22 | 7–11 wk old + 7 mo infection | 15 males, 12 females | INS-GAS FVB/N (USA) | HP SS1 infected | Firmicutes, Bacteroidetes, Proteobacteria | 3 ASF species + H. pylori | Increased Firmicutes (Lactobacillus) and decreased Firmicutes (Clostridium) and Bacteroidetes (Bacteroides). H. pylori = 0.55% of bacteria in all mice. Increased pathology ~increased bacterial load |
*
N/A, Not available