Table 1. Sequence and position of Primer pairs used for PCR and Sequencing.
Primer Name | Primer Sequence | Polarity | Region |
5′ UTR | TTAAAACAGCTCTGGGGTTG | Sense | 5′UTR |
241c | TACTGGGCTTTTCGAAGTAC | Antisense | 5′UTR |
UG53 | TGGCTGCTTATGGTGACAAT | Sense | 5′UTR |
921c | CGTCCTTAATGGGTTCGGTG | Antisense | 5′UTR |
1737 | GTGTWGTGAGTTCAA[Y]GG | Sense | VP4 |
UC24 | ATCATGGTGTCTATTTCTGC | Antisense | VP3 |
UG1 | TTTGTGTCAGCGTGTAATGA | Sense | VP1 |
UC1 | GAATTCCATGTCAAATCTAGA | Antisense | VP1 |
Y7 | GGGTTTGTGTCAGCCTGTAATGA | Sense | VP3 |
Q8 | AAGAGGTCTCTRTTCCACAT | Antisense | 2A |
PV1A | TTIAIIGCRTGICCRTTRTT | Antisense | VP1 |
PV4S | ACITAYAARGAYACIGTICA | Sense | VP1 |