| Xist |
PRC2 |
Co-localization by immunostaining (antibodies α-EZH2, and α-EED),34 RIP-qPCR (antibodies α-EZH2, and α-Suz12),26 RIP-Chip27
|
| Kcnq1ot1 |
G9a and PRC2, Dnmt1 |
RIP-qPCR (antibodies α-G9a, α-EZH2, and α-Suz12),35 RIP-qPCR (antibody α-Dnmt1)36
|
| Air |
G9a |
RIP-qPCR (antibody α-G9a)37
|
| HOTAIR |
PRC2 and LSD1-CoREST |
RIP-qPCR (antibodies α-LSD1 and α-EZH2),31,33 in vitro transcribed biotinylated RNA pull-down with cell extracts or purified PRC2 and LSD1 complexes,31,33 RIP-Chip27
|
| ANRIL |
PRC1 (CBX7) and PRC2 |
RIP-qPCR (CBX7), RNA EMSA, biotinylated RNA pull-down,38 RIP-qPCR (antibody α-Suz12)39
|
| pRNA |
Dnmt3b |
Southwestern blot and pull-down experiments40
|
| Mistral |
MLL-Trx (MLL1) |
RIP-qPCR (MLL1), Recombinant MLL1 - in vitro binding assays41
|
| HOTTIP |
MLL-Trx (WDR5) |
RIP-qPCR (antibody α-WDR5), Recombinant GST-WDR5 - in vitro transcribed RNA interaction42
|
| DBE-T |
MLL-Trx (Ash1L) |
RIP-qPCR (antibody α-Ash1L), Recombinant GST-Ash1L - in vitro transcribed RNA interaction43
|
| PINC |
PRC2 |
RIP-qPCR (antibodies α-EZH2, α-Suz12 and α-RbAp46)44
|
| PTENpg1 asRNA α |
Dnmt3a |
RIP-qPCR (antibody α-Dnmt3a)45
|
| Fendrr |
PRC2 and MLL-Trx (WDR5) |
RIP-qPCR (antibodies α-EZH2, α-Suz12, and α-WDR5)9
|
| Braveheart |
PRC2 |
RIP-qPCR (antibody α-Suz12), Biotinylated RNA pull-down11
|
| NeST |
MLL-Trx (WDR5) |
RIP-qPCR (FLAG-WDR5 transfection and IP with antibody α-FLAG)46
|
| UBC1 |
PRC2 |
RIP-qPCR (antibodies α-EZH2, and α-Suz12)47
|
| Pint |
PRC2 |
RIP-qPCR (antibody α-Suz12), in vitro transcribed biotinylated RNA pull-down with cell extracts or purified PRC248
|
