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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Biomed Mater Res A. 2013 Aug 8;102(7):2173–2180. doi: 10.1002/jbm.a.34902

Figure 5.

Figure 5

Histology Montage of Day 35 Time-point. Left column: Montage of IHC for CD68 to assess macrophage infiltration. Center column: TUNEL stain to assess apoptotic cells. For both IHC CD68 and TUNEL analysis, twenty digital images were captured at 400× magnification for each tissue sample, n=4 at time-points 7, 21 and 35 status-post obstruction or sham. Positive cells were averaged per group and ANOVA with Tukey post-hoc was used to determine statistical significance. Right column: Montage of Trichrome stains to assess for fibrosis. Digital images were captured at 40× magnification for the entire area of each tissue sample, n=4 at time-points 7, 21 and 35 days status-post obstruction or sham. Imaging analysis quantified the percent of fibrotic area.