Figure 1. miR-22 is upregulated in Exo^IPC.

A. Electron microscopy was used to image the structure of an exosome, which was approximately 100 = 100 nm. B. Histogram showing exosome size distribution. X values: Vesicle diameter (nm); Y value: frequency of vesicles. C. Equal amounts of proteins from exosomes and culture medium were analyzed by western blotting for the exosome-enriched protein, CD63. D. RNA analysis by bioanalyzer showed no ribosomal RNA 28 s and 18 s presence. E. microRNA microarray data showing upregulation of miRs in Exo^IPC as compared to Exo^non-IPC. Exo^IPC exosomes from MSCs following ischemic preconditioning. Exo^non-IPC: exosomes from MSCs without ischemic preconditioning. FC: fold change (Exo^IPC vs. Exo^non-IPC). F. qPCR was performed to confirm the enrichment of miR-22 in exosome^IPC as compared to exosome^non-IPC. cel-miR-39 was used as invariant control. (*) denotes P<0.05 for significant difference (n = 3). Exo^IPC exosomes from MSCs following ischemic preconditioning.