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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Biomol NMR. 2013 Dec 20;58(1):69–81. doi: 10.1007/s10858-013-9806-y

Fig. 3.

Fig. 3

Example of the assignment of the isoleucine resonances of MerF by the method of heteronuclear dipolar coupling correlated isotropic chemical shift analysis. A., B., C., and D. are two-dimensional spectra of MerF in q=3.2 14-O-PC/6-O-PC bicelles. A. and B. are “flipped” with the bilayer normal parallel to the field; and C. and D. are “unflipped” with the bilayer normal perpendicular to the field. A. and C. are SLF spectra. B. and D. are HETCOR spectra. The assignment derived from combined DCCICS and PISA wheel analysis are labeled in spectra C and D. Insets in D are extracted from three-dimensional HETCOR/SLF spectrum of the same sample to occupy the missing peaks in two-dimensional spectrum due to selective magnetization transfer.