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. 2014 Feb 18;9(2):e88840. doi: 10.1371/journal.pone.0088840

Figure 4. A nick occurring in asynchronous or G1 arrested cells stimulates gene correction by oligonucleotides equally efficiently.

Figure 4

Shown are frequency of Trp+ transformants by oligonucleotides when cells were asynchronous (left) or arrested in G1 (right) at the time of wild-type-I-SceI or K223I I-SceI breakage prior to oligonucleotide transformation and when the SSB is generated on the “Crick” strand. All data are presented as the median with range (n≥5). For the specific numerical values see Table S3E. Wild-type I-SceI strains used: SAS-227 and SAS-228. K223I I-SceI strains used: SAS-229 and SAS-230. D145A I-SceI strains used: SAS-231 and SAS-232.