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. 2014 Feb 18;9(2):e88840. doi: 10.1371/journal.pone.0088840

Figure 5. K223I I-SceI SSB can stimulate recombination in human cells.

Figure 5

(A) Scheme showing disrupted RFP plasmid locus and disrupted GFP plasmid or chromosomal locus containing the I-SceI recognition sequence (black box). The position of the SSB is indicated by a black line. Dashed gray lines indicate the complementarity between the F oligonucleotide and the antisense strand of the targeted gene, and between the R oligonucleotide and the sense strand of the targeted gene. (B–D) Frequency of fluorescent cells following expression of wild-type I-SceI (dark blue bars labeled “DSB”), K223I I-SceI (orange bars labeled “SSB”), or D145A I-SceI (yellow bars labeled “Non-break”) using either of the single oligonucleotides to repair the break. All data are presented as the median with range. For the specific numerical values see Table S3F-H. (B) Recombination at the RFP target plasmid locus (n = 6). (C) Recombination at the GFP target plasmid locus (n = 9). (D) Recombination at the GFP target chromosomal locus (n≥8).