Figure 6. Folic acid reduces superoxide production, restores eNOS function, improves NO production, and increases aortic H₄B levels in Ang II-infused apoE null mice.

A) Total superoxide production measured from aortic homogenates using electron spin resonance (ESR). eNOS uncoupling activity was assessed by comparing measurements with and without the addition of L-NAME, a NOS inhibitor (n = 6). A reduction in superoxide production with L-NAME indicates that NOS is uncoupled and producing superoxide, while an increase in superoxide production with L-NAME indicates that NOS is coupled and producing NO. B) NO production measured from aortas using ESR (n = 5–6). C) Total H₄B levels measured from aortas using HPLC (n = 5). *p<0.05, #p<0.001.