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. 2014 Feb 18;9(2):e88868. doi: 10.1371/journal.pone.0088868

Figure 2. Decreased expression of the γ-glutamylcysteine ligase and increased hepatic oxidant generation and protein oxidative damage at Day+4 in Allo BMT.

Figure 2

Panel A: At post-transplant day 4, hepatocytes from Syn (B6→B6) and Allo (Balb/C→B6) mice (N = 4 per group) were freshly isolated and the mRNA transcripta of the catalytic sub-unit of γ-glutamylcysteine ligase (GCLC; Green, left axis), were quantified by RT-PCR and normalized to 18S mRNA levels. Cellular reactive oxygen species (ROS) levels were estimated by flow-cytometric detection of CellRox Deep Red Reagent (CDRR) fluorescence. Results are Mean±SD. * = p<0.05. Panel B: Hepatic protein oxidative damage levels in non-transplanted controls (Day 0; N = 4), Syn (B6→B6; N = 3 per time point; Gray bar) and Allo (Balb/C→B6; N = 5 per time point; Black bar) BMT mice were measured by protein carbonyl ELISA assay as described in methods. Results are mean±SD. * = p<0.05 and ** = p<0.01.