Table 1. Description of Experimental BMT Model.
Donor Strain | Recipient Strain | Mismatch Type | Conditioning Regimen | Major T-cell Type | Cell Type & Dosage | |
Model 1. GVHD with Conditioning Regimen | ||||||
Syngeneic (Syn) | C57/Bl6(Thy1.1: H-2b) | C57/Bl6(Thy1.2: H-2b/CD45.2+) | 1100 cGy | CD4+ and CD8+ | TCD-BM cells (10×106) with CD4+ | |
Allogeneic (Allo) | Balb/C(H-2d/CD45.2+) | C57/Bl6(Thy1.2: H-2b/CD45.2+) | MHC-I, -II,and miHAs | 1100 cGy | CD4+ and CD8+ | TCD-BM cells (10×106) with CD4+ |
Model 2. GVHD without Conditioning Regimen | ||||||
Syngeneic (Syn) | C57/Bl6(Thy1.1: H-2b/CD45.2+) | C57/Bl6(Thy1.2: H-2b/CD45.2+) | None | Whole Splenocytes (50×106 cells) | ||
Allogeneic (Allo) | C57/Bl6(Thy1.1: H-2b/CD45.2+) | B6D2F1(H-2bxd, CD45.2+,Thy1.2+) | MHC-I, -II,and miHAs | None | CD4+ and CD8+ | Whole Splenocytes (50×106 cells) |
MHC – Major histocompatibility complex.
MiHAs – Minor histocompatibility antigens.