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. 2014 Feb 18;9(2):e89013. doi: 10.1371/journal.pone.0089013

Table 2. Number of samples available and analysed with each detection method.

Pneumococcal conventional methods Pneumococcal additional detection methods Detection of other pathogens
Blood culture Pleural fluid culture Pleural fluid PCR Blood SSS Blood rtPCR NPA viral culture NPA viral and atypical PCR
Available samples, n (%) 538 (95.9) 53 (47.3) a 53 (47.3) a 383 (71.0) 519 (96.3) 193 (35.3) 193 (35.3)
Samples analysed, n (%) 538 (100.0) 49 (92.5) 22 (41.5) 171 (45.2) 154 (29.7) 163 b (82.3) 160 b (80.8)

NPA, nasopharyngeal aspirate or swab; PCR, polymerase chain reaction; rtPCR, real-time polymerase chain reaction; SSS, serotype-specific serology on paired serum samples.

a

proportion of patients with pleural effusion (N = 112).

b

number of samples that were analysed by each method;130/193 samples were analysed by both methods.