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. 2014 Feb 18;9(2):e89004. doi: 10.1371/journal.pone.0089004

Table 1. Statistical analysis of the protection effect of lactic acid-producing bacterial isolates on C. elegans infected with ETEC JG280a.

P-valuef
Treatmentb Originc Putative identityd Survival (%) 95% CI (± %) DT50e (day) L+J vs E. coli OP50 L+J vs E+J
E+JG280 0.00 4.67 3.70 <0.0001
E. coli OP50 91.11 5.67 >10 <0.0001
CL10 P Lactobacillus acidophilus 39.47 8.13 6.03 <0.0001 <0.0001
CL11 P Lactobacillus casei 23.26 6.34 3.78 <0.0001 0.046
CL12 P Lactobacillus amylovorus 39.02 7.91 7.52 <0.0001 <0.0001
K16 P Pediococcus pentosaceus 67.74 3.21 >10 0.004 <0.0001
K30 P Lactobacillus sp. 35.71 8.26 6.01 <0.0001 <0.0001
K67 P Lactobacillus reuteri 30.77 3.12 4.99 <0.0001 0.006
S8 P Lactobacillus plantarum 34.88 5.41 5.34 <0.0001 0.001
S20 P Lactobacillus johnsonii 21.82 3.63 5.28 <0.0001 0.001
S33 P Pediococcus pentosaceus 70.00 5.94 >10 0.005 <0.0001
S64 C Lactobacillus reuteri 77.42 5.75 >10 0.049 <0.0001
S65 P Lactobacillus plantarum 61.29 11.17 >10 <0.001 <0.0001
S66 P Lactobacillus plantarum 56.82 10.12 >10 <0.0001 <0.0001
LB1 C Lactobacillus zeae 78.57 7.28 >10 0.093 <0.0001
Heat-killed LB1 22.12 5.67 >10 <0.0001 0.047
a

Summary of two or more separate experiments. Survival of worms on the last day (day 10) of the assays with 95% confidence interval (CI) was estimated with the Kaplan-Meier survival analysis.

b

E+JG280: treatment with E. coli OP50 and then with ETEC K88 strain JG280. In the assays with LAB isolates, the nematode was firstly treated with a LAB isolate and then with ETEC JG280.

c

C, isolates from chickens; P, isolates from pigs.

d

Putative species identity was determined by BLAST analysis of sequences of 16S rRNA genes. Sequence similarities between the isolates and the 16S rDNA database sequences were 98 to 100%. Among the thirteen isolates, CL10, CL11, CL12, S64 and LB1 have been reported previously (7).

e

DT50, the time at which half of the worms were dead.

f

Comparisons of survival curves. L+J, C. elegans was treated with Lactobacillus and then JG280. L+J vs E. coli OP50, the statistical difference between the group of C. elegans treated with Lactobacillus followed by JG280 and the group of C. elegans treated with E. coli OP50 only (control group). L+J vs E+J, the statistical difference between the group of C. elegans treated with Lactobacillus followed by JG280 and the group of C. elegans treated with E. coli OP50 followed by JG280.