Figure 6. Rpl5 mutant ES cells exhibit more severe growth defect than Rps19 mutant cells.

Cells were seeded in 6 well plates at a concentration of 5×10³ per well with ES maintenance media, and live cell counts were performed daily for 5 days using Trypan blue. The total number of cells from the two mutants were normalized to their respective parental line and represented as a percentage. From days 3–5 of culture, the Rpl5 mutant ES cells expanded at a significantly slower rate, when compared with the Rps19 mutant ES cells (three independent pooled experiments).