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. 2014 Feb 18;9(2):e89195. doi: 10.1371/journal.pone.0089195

Figure 4. Effect of mutations in the N-terminal F/YxxF/Y motifs of DCAF1 on Vpr and DDB1 binding.

Figure 4

A. HEK293T cells were mock-transfected (lanes 1 and 2) or transfected with Myc-DCAF1 WD (lanes 3 and 4), Myc-DCAF1 WD F1060A/Y1063A (lanes 5 and 6), Myc-DCAF1 WD F1077A/F1080A (lanes 7 and 8) or with Myc-DCAF1 WD Y1120A/F1123A (lanes 9 and 10)-encoding plasmids in the presence of empty vector (lanes 1, 3, 5, 7 and 9) or HA-Vpr-expressing plasmid (lanes 2, 4, 6, 8 and 10). Immunoprecipitations and Western Blot detection were performed as described in figure 2B. * denotes the light chain of the IgG used for immunoprecipitation. B. Quantitation of the DDB1 and HA-Vpr binding. Quantitation was determined as described in figure 2C.