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. 2014 Feb 18;9(2):e89150. doi: 10.1371/journal.pone.0089150

Figure 4. VEGF secretion.

Figure 4

VEGF secretion was investigated in RPE/choroid organ culture (A) and ARPE-19 cell culture (B). RPE/choroid perfusion organ cultures were treated with 100 µg/ml fucoidan for 3 days and supernatant was collected at 6 hours, 24 hours and 3 days for one hour. ARPE-19 cells were treated with 100 µg/ml fucoidan for five days, and medium was collected after 1 day, 3 days and 5 days. VEGF content was evaluated with ELISA. Fucoidan reduced VEGF content compared to control in organ culture after 24 hours and 3 days (A). In cell culture, a reduction of VEGF secretion can be found after 3 days and 5 days (B). Significance was determined with student's t-test, + p<0.05; ++ p<0.01.