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. 2014 Feb 18;9(2):e89399. doi: 10.1371/journal.pone.0089399

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© 2014 Makrantoni et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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VMY357 (SLI15-EGFP), SJC591 (sli15-20A) and VMY375 (sli15-20D) cells containing mCherry-TUB1 and pMET3-CDC20 were arrested in G1 with α-factor at 26°C and then released to a metaphase block in rich medium for 2.5 h. (A) Representative images of live cells arrested in metaphase. (B) Quantification of spindle associated Sli15-EGFP fluorescence in cells from multiple fields imaged 2.5–3.5 h following α-factor release. The top and bottom of the boxes indicate the 75^th and 25^th percentiles and the whiskers indicate the 10^th and 90^th percentiles. Significance levels were determined using the Mann-Whitney U test of the null hypothesis that the medians were identical. SLI15 vs. sli15-20A: Mann-Whitney U = 36165, n_SLI15 = 378, n_sli15-20A = 387, p<0.001, two tailed. sli15-20D vs. SLI15: Mann-Whitney U = 15992, n_sli15-20D = 381, n_SLI15 = 378, p<0.001, two tailed. Data shown are representative of three separate experiments. AU, arbitrary units of fluorescence.