Table 1. Yeast Strains.
| Strainsa | Genotype | Source |
| K699 | MATa | Kim Nasmyth |
| SJC591 | MATa sli15Δ::KanMX6 his3::SLI15-S20A-EGFP::HIS3 trp1::pTEF1-mCherry-TUB1::TRP1 cdc20::pMET-CDC20::TRP1 | This study |
| SJC594 | MATa ipl1(S50A,S76A)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(S335A)::HIS3 | This study |
| SJC597 | MATa ipl1(S50A,S76A)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(20A)::HIS3 | This study |
| SJC600 | MATa ipl1(S50E,S76E)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(S335D)::HIS3 | This study |
| SJC603 | MATa ipl1(S50E,S76E)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(20D)::HIS3 | This study |
| SJC641 | MATa ipl1(S50A,S76A)::HIS3MX6 sli15Δ::KANMX6 his3::SLI15::HIS3 | This study |
| SJC644 | MATa ipl1(S50E,S76E)::HIS3MX6 sli15Δ::KANMX6 his3::SLI15::HIS3 | This study |
| SJC649 | ipl1(S50A,S76A)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(20A,S335A)::HIS3 | This study |
| SJC655 | ipl1(S50E,S76E)::HIS3MX6 sli15Δ::KANMX6 his3::sli15(20D,S335D)::HIS3 | This study |
| VMY30 | MATa sli15Δ::KANMX6 his3::SLI15::HIS3 | This study |
| VMY92 | MATa CFIII (CEN3.L.YPH278) URA3 SUP11 sli15Δ::KANMX6 his3::SLI15::HIS3 | This study |
| VMY148 | MATa sli15Δ::KANMX6 his3::SLI15-S20A::HIS3 | This study |
| VMY157 | MATa CFIII (CEN3.L.YPH278) URA3 SUP11 sli15Δ::KANMX6 his3::SLI15-S20A::HIS3 | This study |
| VMY162 | MATa sli15Δ::KANMX6 his3::SLI15-S20A::HIS3 pds1::PDS1-myc18 ::LEU2 | This study |
| VMY166 | MATa pds1::PDS1-myc18::LEU2 scc1Δ::TRP1 leu2::LEU2::pGAL-SCC1 sli15Δ::KANMX6 his3::SLI15-S20A::HIS3 | This study |
| VMY187 | MATa sli15Δ::KANMX6 his3::SLI15-S20D::HIS3 | This study |
| VMY191 | MATa sli15Δ::KANMX6 his3::SLI15-S20D::HIS3 pds1::PDS1-myc18 ::LEU2 | This study |
| VMY194 | MATa sli15Δ::KANMX6 his3::SLI15::HIS3 PDS1-myc13::NatRMX6 | This study |
| VMY222 | MATa pds1::PDS1-myc18::LEU2 scc1Δ::TRP1 leu2::LEU2::pGAL-SCC1 sli15Δ::KANMX6 his3::SLI15::HIS3 | This study |
| VMY224 | MATa CFIII (CEN3.L.YPH278) URA3 SUP11 sli15Δ::KANMX6 his3::SLI15-S20D::HIS3 | This study |
| VMY316 | MATa sli15Δ::KANMX6 his3::SLI15::HIS3 cdc20::pMET-CDC20::TRP1 CEN5-tetO336::HIS3 leu2::tetR-GFP::LEU2 ura3::VENUS-TUB1::URA3 | This study |
| VMY318 | MATa sli15Δ::KANMX6 his3::SLI15-S20A::HIS3 cdc20::pMET-CDC20::TRP1 CEN5-tetO336::HIS3 leu2::tetR-GFP::LEU2 ura3::VENUS-TUB1::URA3 | This study |
| VMY320 | MATa sli15Δ::KANMX6 his3::SLI15-S20D::HIS3 cdc20::pMET-CDC20::TRP1 CEN5-tetO336::HIS3 leu2::tetR-GFP::LEU2 ura3::VENUS-TUB1::URA3 | This study |
| VMY356 | MATa pds1::PDS1-myc18::LEU2 scc1Δ::TRP1 leu2::LEU2::pGAL-SCC1 sli15Δ::KANMX6 his3::SLI15-S20D::HIS3 | This study |
| VMY357 | MATa sli15Δ::KANMX6 his3::SLI15-EGFP::HIS3 trp1::pTEF1-mCherry-TUB1::TRP1 cdc20::pMET-CDC20::TRP1 | This study |
| VMY361 | MATa sli15Δ::KANMX6 his3::SLI15-S335D::HIS3 | This study |
| VMY363 | MATa sli15Δ::KANMX6 his3::SLI15-S335A::HIS3 | This study |
| VMY365 | MATa sli15Δ::KANMX6 his3::SLI15-S20A-S335A::HIS3 | This study |
| VMY367 | MATa sli15Δ::KANMX6 his3::SLI15-S20D-S335D::HIS3 | This study |
| VMY375 | MATa sli15Δ::KANMX6 his3::SLI15-S20D-EGFP::HIS3 trp1::pTEF1-mCherry-TUB1::TRP1 cdc20::pMET-CDC20::TRP1 | This study |
| VMYD16 | MATa/MATα SLI15/sli15Δ::KANMX6 | This study |
a
All strains are in the W303 background: ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 ssd1-d2 Gal+.