Table 4. Comparison of in vivo and in vitro Ipl1 phosphorylation sites in Sli15.
| Observed a , b | Mutated in sli15-20A and sli15-20D b | Mutated in sli15-17A c | Detected in vivo d | Sequence contexte |
| 137 | - | RFSI | ||
| 177 | 177 | RESS | ||
| 233 | 233 | 233 | RRSN | |
| 256 | - | KSSG | ||
| 268 | 268f | KESP | ||
| 276 | 276 | 276 | 276 | KKST |
| 277 | 277 | - | KSTI | |
| 298 | 298 | 298 | - | KASS |
| 305 | 305 | 305 | - | RLSS |
| 317 | - | KISR | ||
| 326 | 326 | 326 | - | KYSS |
| 341 | - | KVSQ | ||
| 382 | 382 | 382 | 382 | RKSS |
| 383 | 383 | 383 | 383 | KSSI |
| 391 | - | KTSL | ||
| 395 | - | KLTT | ||
| 413 | - | KHSS | ||
| 432 | 432 | KISV | ||
| 457 | 457 | - | RPTK | |
| 460 | 460 | 460 | - | KASI |
| 489f | KLSP | |||
| 509 | 509 | - | RLTN | |
| 533 | 533 | RLSG | ||
| 554 | 554 | 554 | RMSH | |
| 564 | 564 | 564 | - | KQTS |
| 578 | 578 | 578 | 578 | KESL |
| 589 | 589 | 589 | - | RDTI |
a
See Table 3 for phosphorylation site mapping data.
b
This study.
c
Nakajima et al. [26]; these sites represent all occurrences of the motif [KR]-X-[ST]-[ILVST] in Sli15.
d
All verified in vivo phosphorylation sites present in the PhosphoGrid [50], [51] and Phosphopep databases [52] that have either K or R in the −2 position as required for potential Aurora B/Ipl1 phosphorylation, but without restricting the identity +1 residue.
e
Phosphorylated residue is shown in bold.
f
Possible in vivo target of a proline-directed protein kinase such as Slt2 or Cdc28 rather than Ipl1.