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. 2014 Feb 18;9(2):e89435. doi: 10.1371/journal.pone.0089435

Figure 4. Morphological changes in pupal wings induced by injection of ecdysteroid (20-hydroxyecdysone, 20E).

Figure 4

(A) Female-pupal wing without application of 20E on Day 6 after injection of 6 µl (4.7 µg) ethanol. Female-pupal wing on Day 6 (B), Day 8 (C), and Day 11 (D) after injection of 5.4 µg 20E. (E) Male-pupal wing after injection of 6 µl (4.7 µg) ethanol. Male-pupal wing on Day 6 (F), Day 8 (G), and Day 11 (H) after injection of 5.4 µg 20E. All wing epithelia are attached to their pupal-wing case. Arrows point the distal end of the degenerating wing epithelium in the female (B–D). Scale bar  = 1 mm.