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. 2014 Feb 18;9(2):e89435. doi: 10.1371/journal.pone.0089435

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© 2014 Niitsu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The pupal-wing epithelium of females (A–D) and males (E–H). Pupal wing epithelium of females (A) and males (E) on Day 6 after injection of 6 µl ethanol. Pupal-wing epithelium of females on Day 6 (B), Day 8 (C) and Day 11 (D) after injection of 5.4 µg 20E. Phagocytes (white arrows) and apoptotic-body-like structures (black arrows) are visible (D). Pupal-wing epithelium of males on Day 6 (F), Day 8 (G) and Day 11 (H) after injection of 5.4 µg 20E. Scale is visible in the pupal epithelium (F, G, H). BL, basal lamina; P, phagocyte; S, scale; T, trachea; t, tracheole. Scale bar = 20 µm.